SentiGuide allows a preoperative mapping of the sentinel lymph node to enable needle biopsy. This is achieved by augmenting a high-resolution 3D SPECT image on the ultrasound image and then performing an ultrasound guided needle biopsy instead of extracting the sentinel lymph node by means of surgery.

The SentiGuide generates SPECT images using a highly sensitive handheld gamma camera combined with an optical navigation system. By scanning around the region of interest, data is captured and used to reconstruct a high-resolution SPECT image.